白色杜洛克×二花脸资源家系猪血液性状的系统遗传学研究

doi:10.11843/j.issn.0366-6964.2016.02.004

Abstract

Abstract:

To dissect the hereditary basis for hematological traits，we integrated the results of genome-wide association study and muscle expression quantitative loci(eQTL) analysis to identify the candidate genes in a White Duroc×Erhualian pigs F₂ resource population on the basis of systems genetics.In this study，the 18 hematological parameters of 1 149 pigs were measured and the expressed transcripts of muscles in 593 pigs were detected，1 020 individuals were genotyped.The correlations between gene expressions and phenotypic data were evaluated using Spearman correlation coefficient.Those eQTL were identified by aligning the related transcripts with the pig reference genome and their ontology enrichment were also implemented.We also performed the integrated analysis by incorporating eQTL information into our blood-based GWAS data.In this study，122 eQTLs were identified including 9 cis-eQTLs and 63 trans-eQTLs with a conservative threshold P<5×10^-4.Of them，SERPINB6 was an identified candidate gene by eQTL.PTPRC and ITGA8 were prioritized as candidate genes by gene ontology enrichment analysis.TMED9，PCK1，TINAGL1 and SORL1 were highlighted as the most promising candidate genes by integrative eQTL and GWAS analysis.We identified 7 novel candidate genes by integrating the previous blood-based genome-wide association study and muscle gene expression profiles analysis.PCK1，TMED9，TINAGL1 and SORL1 shared the association signals of GWAS and eQTL.

CLC Number:

S828
S813.3

XU Pan，ZHANG Zhen，CUI Lei-lei，YANG Bin，DUAN Yan-yu. A Systems Genetics Study of Hematological Traits in a White Duroc × Erhualian Pigs F₂ Resource Population[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 232-240.

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A Systems Genetics Study of Hematological Traits in a White Duroc × Erhualian Pigs F₂ Resource Population

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A Systems Genetics Study of Hematological Traits in a White Duroc × Erhualian Pigs F2 Resource Population

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A Systems Genetics Study of Hematological Traits in a White Duroc × Erhualian Pigs F₂ Resource Population